LDH Purification lab Report Essays

LDH Purification lab Report Essays LDH Purification lab Report Paper LDH Purification lab Report Paper OLD was purified from the ammonium sulfate precipitated protein mixture by affinity chromatography and its activity was studied by spectrophotometers determination of NADIA at 340 NM. From Pierce BCC assay of crude homogenate, initial protein concentration was shown to be 100 MGM/ml. The final protein concentration of the pooled affinity sample was shown to be 0. 2 MGM/ml. It was found that the total specific activity of OLD was 58. 5 mol/min/MGM, and yield of 0. 6%. Even though we were successful in purifying OLD enzyme, further steps can be taken to increase the yield. Materials and Methods Cell Lysine and Extraction of OLD: Approximately 40 g of minced chicken breast eat (40. 327 g) is blended with ml cold extraction buffer in four 30-seconds bursts for homogenate of the muscle tissue. The extraction buffer contained mm Tries-HCI (pH-7. 4), mm 2-Merchantable, mm Phenylmethylsulfonylflouride (AMPS), 1 mm Ethylene Dianne attracted acid (EDIT). The homogeneities procedure was carried out in the cold room to prevent the denomination of proteins. The homogenate was centrifuged at 15,000 RPM for 20 minutes at 40 C. The supernatant was filtered through two layers of cheesecloth to remove lipids from the supernatant. The total volume was noted and three 0. Ml aliquots (crude extract) were stored at -200 C. Ammonium sulfate precipitation: 60% ammonium sulfate concentration was used to precipitate proteins. 0. 39 g of ammonium sulfate per ml of the supernatant was added gradually to the supernatant for 15-20 min with continuous gentle stirring at 40 C. The mixture was centrifuged for 20 minutes at 1 5,000 RPM at 40 C. The supernatant was discarded and the pellet was stored at -200 c. Dialysis: Ammonium precipitation leads to high concentration of salts in protein mixture that can interfere with further purification steps. In order to remove excess salts, dialysis was performed. The pellet was suspended in Tries-AMPS buffer (10 rim Tries-HCI, pH 8. 6, 0. 5 mm 2-Merchantable, and mm ratio of EDIT) and mixed very gently until it dissolved at 40 C. Volume of ml protein mixture was added in the dialysis tubing and incubated twice overnight with two IL buffer changes (Same buffer as extraction buffer that was used for cell lysine). After two incubation, protein mixture was responded gently and centrifuged for 10 minutes at 15,RPM at ICC. Pellet was discarded, total volume of supernatant was noted and three 0. 1 ml aliquots were collected. Affinity Chromatography: Isobaric Blue column was used to separate OLD from the other proteins. Ml fractions were collected in thirteen test tubes. Column was first rinsed with Tries-AMPS buffer followed by addition of protein mixture. Then, ml AND Buffer (mm Tries-HCI pH-8. 6, 0. Mm 2-Merchantable, mm Lithium acetate and 1 mm AND+) was added followed by NADIA (mm Its- HCI PH 8. 6, mm NADIA and 0. Mm 2-Merchantable). Between each steps, column was washed with ml Tries-AMPS Buffer. Each fraction was subjected to absorbency reading of Mann. For absorbency above 1. NM, 1:10 dilutions were carried out. Activity Assay: We used OLD Enzyme assay to measure the amount of OLD activity in our protein mixture. OLD catalysts the conversion of lactate to private and AND+ to NADIA. The NADI A can be determined spectrophotometers at 340 NM. The OLD assay was performed in the crude homogenate, desalted fraction and six peak fractions from the Isobaric blue column. A cocktail solution was prepared by mixing lactate stock solution (120 rim lithium lactate, 10 mm Tries-HCI; pH 8. 6), AND+ stock solution (12 mm AND+, 10 mm Tries HCI; pH 8. 6) and bicarbonate stock solution (18 mm Enhance, 0. 5 M Nasal) in the ratio of in cavetti. 0 micrometers of the sample is then added and the assay absorption is measured at Mann. If absorbency was above 1. 5, samples were diluted. Protein Assay: The Pierce BCC Protein Assay (Thermo Scientific) is a detergent- compatible formulation based on bioscience acid (BCC) for the colorimetric detection and quantization of total protein concentration. A series of standard solution of Bovine Serum Albumin (BAS) ranging from 0-2000 pig/ml was prepared from a stock solution of 2 MGM/ml BAS. Lull of diluted crude (1:500, 1 :250), desalted (1:100, 1:50), and 6 peak fractions from isobaric blue column (1:10, 1:5) ere loaded in microscope along with lull of BCC working reagent. Microscope was incubated for mini at ICC and then the absorbency was measured at Mann. Results/Discussion The purpose of this experiment was to extract and purify OLD enzyme from chicken muscle tissue using a variety of techniques including homogeneities, ammonium sulfate precipitation, dialysis, and affinity chromatography. Activity and Protein assay were used to track the overall amount of OLD present in the samples. Crude Extraction: Chicken muscle tissue was homogeneity in a blender with cold extraction buffer in order to else cells, releasing OLD into slurry of tissue monuments. Centrifugation separated membranes, nuclei, and other large cellular components to a pellet leaving a supernatant of crude product. Controlling temperature was a major consideration after homogeneities since not only did this step releases proteins like OLD from the cell, but it also releases proteases that can now interact to degrade the OLD. Keeping samples on ice, pre-cooling the buffer, and avoiding excess kinetic energy through conservative blending were methods used to minimize activity of these proteases. After filtration through cheesecloth, our final volume of crude homogenate sample ml, much more volume than expected. Addition of more than ml of buffer volume could have increased the volume. Other possible explanation is that more solid components such as fats were present in the sample and hence, more than 20 minutes of centrifugation was required. Desalted Sample: 60% ammonium sulfate is added to the crude extract that precipitates OLD proteins. The resulting 40% pellet theoretically contains most of the original OLD, which is re-suspended in very less volume (ml) to create a more concentrated sample. This process leads to high concentration of salts in rotten mixture that can interfere with subsequent purification steps. Ml protein mixture underwent dialysis procedure that removes excess salts and our final volume after dialysis was ml. One possible explanation for increase in our volume could be that extraction buffer got mixed with protein mixture either due to tubing leaking or tubing clips not being properly tightened. Affinity Chromatography: Isobaric Blue column is an affinity column, which is specific to dehydrogenate type proteins, due to a compound structurally similar to NADIA being attached covalently attached to the column. 13 fractions were elected and absorbency was measured at Mann to check presence of OLD protein in the fractions. 1:10 dilution was performed if absorbency reading was above 1. NM since it spectrographically indicates saturation and less than 1% light reaching the detector. During the addition of protein mixture (fraction# 4), high absorbency reading of NM was obtained (Fig. 1). This could be due to lot of non-dehydrogenate-type proteins present in our sample that got eluted first during affinity chromatography. Second peak was seen after AND+ was added since AND solution results in the removal of the loosely bound protein. Third peak was seen after NADIA was added since NADIA solution results in release of maximum OLD proteins (Fig l) Enzyme Activity Assay: The OLD activity was measured spectrophotometers by measuring the absorbency of NADIA at 340 NM. Three peak fractions were selected for this assay based on their absorbency values obtained after adding AND+ (fraction# 6, 7, 8) and other three after adding NADIA in the affinity chromatography step (fraction# 10, 1 1 , 12). A huge activity of 141 mol/min/ml was seen at fraction# 7(PUFF ) which indicated that we had lot of proteins present in our sample. Second peak activity was seen t fraction indicating that more OLD proteins is present in this fraction than in fraction# 11 (PUFF) (fig. 1). Based on this information, we selected fraction #10 as for our protein assay. Desalted showed highest activity among all the samples (Tablet ) possible due to errors occurring during dialysis explained previously. Figure 1. Absorbency readings of eluted obtained from affinity chromatography with OLD activity for 6 peak fractions. The desalted fraction was loaded to the Isobaric blue column and proteins were eluted with Tries-AMPS, AND+ and NADIA wash subsequently. The absorbency at 280 NM of eluted were measured after ACH collected fractions. The OLD activity was calculated from the absorbency values obtained at Mann. Protein Assay: We used BCC Pierce Assay to determine protein concentrations in our protein mixture. BAS standard curve was created for series of dilutions ranging from 0-2000 pig/ml and linear graph equation was used to calculate protein concentrations for the samples (Table 1). Based on Table 1, with each subsequent purification step, protein concentration decreases as sample become more concentrated with only OLD protein. Specific activity should increase and total activity should decrease with very purification step as samples get less and less diluted.

How Long Should a Blog Post Be to Get the Most Traffic and Shares

How Long Should a Blog Post Be to Get the Most Traffic and Shares â€Å"How long should my blog post be?† We hear this question a lot. There’s a ton of advice out there that says everything from 275 to 2,500 words. That’s †¦ not a very helpful range. So, we did some research to definitively determine the answer. The following studies will show you what length of content gives you the best chance of ranking on Google. Plus, we’ve included some raw data we compiled from our own top-performing blog posts (and will show you how to analyze your own top content to determine your own best blog post length). Optimize Your Blog Content With This Template Bundle Dont just guess at the best blog content length. Know the best length for every post on your blog. Snag this template bundle, and youll get: A Blog Post Template to help you write posts that are the optimal length for the best results. A Blog Post Length Analysis Template to record your own data and draw your own conclusions (just like we did at ). ’s 2018 Blog Content Length Research Report. This report breaks down different trends that we have spotted in our content that may influence how you create your own. All that for free? Get em now, and then lets continue.How Long Should a Blog Post Be to Get the Most Traffic and Shares?About The 12 Blog Post Length Studies ... So, where did we get all our information from, anyway? The answer is these twelve studies (plus our own research): For Blog Posts in 2017, What’s the Best Length for SEO from Snap Agency. How Long Should a Blog Post Be from Yoast. Blogging Statistics and Trends: The 2017 Survey of 1000+ Bloggers from Orbit Media. How Long Should Your Blog Post Be? A Writer’s Guide from The Write Practices. How Long Should My Blog Post Be from Forbes. 4 Statistics Every Blogger Should Know About Content Word Count from Copy Press. 2017 State of the Blogging Industry from Convert Kit. Compiled research from Clariant Creative. The Perfect Blog Post Length and Publishing Frequency is B?!!$#à ·x from MOZ. ProBlogger FAQ: How Long Should Posts Be from Problogger. The Optimal Length for Every Social Media Update and More from Buffer. The Character Count Guide for Blog Posts, Videos, Tweets More from Hubspot. Want to know how long blog posts should be? Check out these 12 studies.Check out this infographic for more statistics from the studies: DID YOU KNOW: 's WordPress and social media integrations make it easy to plan, publish, and promote every blog post on one marketing calendar. What Our Data Demonstrates We gathered data on our top blog posts that have gained the most traffic and the most social shares. Here’s what we found. Our Top Traffic Data After analyzing our posts, the following data was found from our top ten highest-traffic posts: What can we draw from the data we found? Every post that ranked on the first page of Google was over 2,000 words. Comprehensive content (which usually needs to be long) ranks well because it helps search engines offer users a complete answer to the query. If the reader doesn't need to bounce back to the search engine to read another post, then the search engine has succeeded. So, help Google, help you. However, just because a post is long, it doesn’t mean it will rank well. The content you write must give your audience what they’re looking for as well as contain relevant keywords and LSI terms. The results we saw prove a lot of what the above studies say is true. If you want your blog content to rank on Google, our data supports the theory that blog posts over 2,000 words tend to outperform shorter content in organic search. Why? The added length also gives you a chance to include the detail you need. This provides more usefulness for the reader which encourages them to stay and read longer.  It also helps ensure that your readers don’t have to bounce back and read multiple posts to gather the information they need. The less readers pogo back to Google, the more likely you'll be rewarded with higher rankings. Our Top Shared Posts After gathering our data on our most shared blog posts, here’s what we found: Interesting data from 's top-shared blog posts ...What conclusions were we able to draw from this data? A specific length doesn’t necessarily mean more social shares.  Take a look at our 5th most shared blog post. It’s only 299 words long. This shows that even though our post was really short (especially by our standards) the topic was popular enough and gave enough information to catch the attention of our audience. The interest of your audience in the topics that you write about may have more of influence on social shares. For example, if you write a 2,000 word post on your love of brussel sprouts that may not go over as well as your 1,000 word post on how to make the best chocolate chip cookies ever. For , Pinterest plays a vital role in the shares our content gets.  Try including an image or two that can be published on the site when you write your next post. Regarding what the previous studies mentioned, we found that while writing at least 1,600 words helped, it didn’t mean that longer posts would necessarily garner more shares. Rather, content that gets shared, is content that's interesting and high-quality (whether it's long or short). Recommended Reading: How to Build the Best Social Media Promotion Schedule For Your Content How To Study Your Own Blog Post Data One way to help determine how long your blog posts should be is to look at and analyze the data from content you have already posted. Remember, your audience will help indicate what kinds of content they want to see. Here are some places to gather your data from. Get Traffic Data From Google Analytics In Google Analytics, you can track the organic and overall traffic from your blog posts by going to Behavior: Site Content: All Pages: Record your traffic for each post in your report template: Track Your Rankings Another critical part of your report is tracking how your content is ranking in Google search engines. There are tons  of tools out there that help you do this (and more). Here are a few: Moz: One of the most popular all-in-one SEO software platforms out there. Ahrefs: This is another popular SEO tool that packs tons of features. SERPs.com: While SERPs offers a robust premium platform, their free rank checker is worth using in a pinch (or on a budget). Another powerful free tool is Google Search Console. While there's a lot you can do with Search Console, not everyone is aware it can pull in ranking data. Watch this video to learn how: Then record the ranking of your posts after thirty days in your report template. Recommended Reading: When is the Best Time to Publish a Blog Post? Check How Often Your Content Is Shared On Social Media The last part of your report is checking the number of social media shares for after the first 30 days that your content has been published. Typically, you would need to go to each social network and check the number of shares on each post you published. Another way to do this is to use ’s Top Content Report. It automatically reports how many shares your content receives.Once you gathered your data, record it in your report (included in this post). As you continue to track your data look for specific trends. Does the word count of your content affect how often your posts are shared? Is your audience clamoring for long-form content posts or do they prefer shorter posts? Use that data to help fuel your blog content creation strategy. DID YOU KNOW: You can discover your most-shared blog posts easily using Top Content Reports in . See how they work here. How Long Should Each Part Of Your Blog Post Be? So far you’ve covered the opinions of 20 different studies, and you’ve seen a brief overview of what the data here at is telling us about the best length of your blog post. But maybe you have a few more questions. After all, a blog post is more than just a few paragraphs of body copy. What are some other components of your blog post that you should keep length in mind for? Here's how long every piece of the perfect #blog post should be:How Long Should Your Headlines Be? Your headline is one of the most important parts of your blog post. It’s what hooks your reader in, and if you don’t grab their attention from the get-go, you’ll lose them before you even begin. So where does the length of your headline come into play? Our research shows that headlines should be about 55 characters  to get the most click-throughs. To test your headlines before you publish your blog posts, use our  Headline Analyzer: How Long Should Your Sub-Headers Be? What’s a sub-header you ask? A sub-header is an H2 or H3 header tag in HTML that breaks up specific sections of each post. An H3 sub-head should be the sub-point of an H2 tag (and an H4 a sub-point of an H3, and so forth). Here at , we stick to H2 and H3-level headers. This helps maintain a clean and crisp appearance. So how long should a standard sub-header be? At we shoot for between eight to 10 words. It gives our readers enough to see there is a break in the section of the post without becoming overly wordy. Source: https://www.copyblogger.com/how-to-write-exquisite-subheads Keep sub-heads between 8 and 10 words long. Learn why (and get more content length tips) here:How Long Should Your Paragraphs Be? For web content writers a short paragraph is another crucial component of keeping your readers engaged with your content. So what’s the best length of a paragraph of text in your blog post? A maximum of three sentences. Why? Because it helps break down huge chunks of text which once again helps the reader consume your content and find the information they’re looking for. If you’re looking for some more insight into web content writing, read this post from Search Engine Journal. Recommended Reading: 40 Content Writing Tips to Make You a Better Marketer Now How Long Should Your Sentences Be? Just like short paragraphs, concise and readable sentences are important for your blog posts. Just like short paragraphs keep your blog post easy to read, so do short sentences. So how short should your sentences be? For easiest readability on the web, the answer is 25 words. Really long sentence have an overly abundant amount of words make it difficult to skim your content, and it becomes really easy to lose your train of thought. ^^See what I mean. Shorter sentences much easier to read. Keep sentences under 25 words for easy readability on the web.How Long Should Your Meta Descriptions Be? What is a meta description? A meta description is the short amount of text that appears below your web page in a Google search. It tells readers at a glance what kind of information is in your post. They look like this: Meta descriptions should be written around 156 characters or less. However, keep in mind that Google recently issued an update, and may display over 230 characters in some (but not all) cases. Summary: Here's How Long Everything in Your Blog Post Should Be We've thrown a lot of data and numbers at you. Feel like your head is spinning? Use this quick cheat sheet: Blog Post Content: 2,500 Words. Title Tag: 70 Characters. Meta Description: 156 Characters. Headlines: 55 Characters (or Six to Seven Words) Sub-Heads: Eight to 10 Words (Or Less) Sentences: 25 Words or Less. Paragraphs: Three Sentences or Less. There you have it! Now Go Write Great Blog Posts To sum up the studies from this post: Long form content is powerful if you want it to rank in Google. However, if your team is looking more for social shares or comments, shorter posts may be the better option for you. No matter what you decide, you need to look at your own data to see what your audience wants. Once you’re ready to start writing, create, edit and publish your blog posts with . Start a trial  or request a demo  today. How Long Should a Blog Post Be to Get the Most Traffic and Shares â€Å"How long should my blog post be?† We hear this question a lot. There’s a ton of advice out there that says everything from 275 to 2,500 words. That’s †¦ not a very helpful range. So, we did some research to definitively determine the answer. The following studies will show you what length of content gives you the best chance of ranking on Google. Plus, we’ve included some raw data we compiled from our own top-performing blog posts (and will show you how to analyze your own top content to determine your own best blog post length). Optimize Your Blog Content With This Template Bundle Dont just guess at the best blog content length. Know the best length for every post on your blog. Snag this template bundle, and youll get: A Blog Post Template to help you write posts that are the optimal length for the best results. A Blog Post Length Analysis Template to record your own data and draw your own conclusions (just like we did at ). ’s 2018 Blog Content Length Research Report. This report breaks down different trends that we have spotted in our content that may influence how you create your own. All that for free? Get em now, and then lets continue.How Long Should a Blog Post Be to Get the Most Traffic and Shares?About The 12 Blog Post Length Studies ... So, where did we get all our information from, anyway? The answer is these twelve studies (plus our own research): For Blog Posts in 2017, What’s the Best Length for SEO from Snap Agency. How Long Should a Blog Post Be from Yoast. Blogging Statistics and Trends: The 2017 Survey of 1000+ Bloggers from Orbit Media. How Long Should Your Blog Post Be? A Writer’s Guide from The Write Practices. How Long Should My Blog Post Be from Forbes. 4 Statistics Every Blogger Should Know About Content Word Count from Copy Press. 2017 State of the Blogging Industry from Convert Kit. Compiled research from Clariant Creative. The Perfect Blog Post Length and Publishing Frequency is B?!!$#à ·x from MOZ. ProBlogger FAQ: How Long Should Posts Be from Problogger. The Optimal Length for Every Social Media Update and More from Buffer. The Character Count Guide for Blog Posts, Videos, Tweets More from Hubspot. Want to know how long blog posts should be? Check out these 12 studies.Check out this infographic for more statistics from the studies: DID YOU KNOW: 's WordPress and social media integrations make it easy to plan, publish, and promote every blog post on one marketing calendar. What Our Data Demonstrates We gathered data on our top blog posts that have gained the most traffic and the most social shares. Here’s what we found. Our Top Traffic Data After analyzing our posts, the following data was found from our top ten highest-traffic posts: What can we draw from the data we found? Every post that ranked on the first page of Google was over 2,000 words. Comprehensive content (which usually needs to be long) ranks well because it helps search engines offer users a complete answer to the query. If the reader doesn't need to bounce back to the search engine to read another post, then the search engine has succeeded. So, help Google, help you. However, just because a post is long, it doesn’t mean it will rank well. The content you write must give your audience what they’re looking for as well as contain relevant keywords and LSI terms. The results we saw prove a lot of what the above studies say is true. If you want your blog content to rank on Google, our data supports the theory that blog posts over 2,000 words tend to outperform shorter content in organic search. Why? The added length also gives you a chance to include the detail you need. This provides more usefulness for the reader which encourages them to stay and read longer.  It also helps ensure that your readers don’t have to bounce back and read multiple posts to gather the information they need. The less readers pogo back to Google, the more likely you'll be rewarded with higher rankings. Our Top Shared Posts After gathering our data on our most shared blog posts, here’s what we found: Interesting data from 's top-shared blog posts ...What conclusions were we able to draw from this data? A specific length doesn’t necessarily mean more social shares.  Take a look at our 5th most shared blog post. It’s only 299 words long. This shows that even though our post was really short (especially by our standards) the topic was popular enough and gave enough information to catch the attention of our audience. The interest of your audience in the topics that you write about may have more of influence on social shares. For example, if you write a 2,000 word post on your love of brussel sprouts that may not go over as well as your 1,000 word post on how to make the best chocolate chip cookies ever. For , Pinterest plays a vital role in the shares our content gets.  Try including an image or two that can be published on the site when you write your next post. Regarding what the previous studies mentioned, we found that while writing at least 1,600 words helped, it didn’t mean that longer posts would necessarily garner more shares. Rather, content that gets shared, is content that's interesting and high-quality (whether it's long or short). Recommended Reading: How to Build the Best Social Media Promotion Schedule For Your Content How To Study Your Own Blog Post Data One way to help determine how long your blog posts should be is to look at and analyze the data from content you have already posted. Remember, your audience will help indicate what kinds of content they want to see. Here are some places to gather your data from. Get Traffic Data From Google Analytics In Google Analytics, you can track the organic and overall traffic from your blog posts by going to Behavior: Site Content: All Pages: Record your traffic for each post in your report template: Track Your Rankings Another critical part of your report is tracking how your content is ranking in Google search engines. There are tons  of tools out there that help you do this (and more). Here are a few: Moz: One of the most popular all-in-one SEO software platforms out there. Ahrefs: This is another popular SEO tool that packs tons of features. SERPs.com: While SERPs offers a robust premium platform, their free rank checker is worth using in a pinch (or on a budget). Another powerful free tool is Google Search Console. While there's a lot you can do with Search Console, not everyone is aware it can pull in ranking data. Watch this video to learn how: Then record the ranking of your posts after thirty days in your report template. Recommended Reading: When is the Best Time to Publish a Blog Post? Check How Often Your Content Is Shared On Social Media The last part of your report is checking the number of social media shares for after the first 30 days that your content has been published. Typically, you would need to go to each social network and check the number of shares on each post you published. Another way to do this is to use ’s Top Content Report. It automatically reports how many shares your content receives.Once you gathered your data, record it in your report (included in this post). As you continue to track your data look for specific trends. Does the word count of your content affect how often your posts are shared? Is your audience clamoring for long-form content posts or do they prefer shorter posts? Use that data to help fuel your blog content creation strategy. DID YOU KNOW: You can discover your most-shared blog posts easily using Top Content Reports in . See how they work here. How Long Should Each Part Of Your Blog Post Be? So far you’ve covered the opinions of 20 different studies, and you’ve seen a brief overview of what the data here at is telling us about the best length of your blog post. But maybe you have a few more questions. After all, a blog post is more than just a few paragraphs of body copy. What are some other components of your blog post that you should keep length in mind for? Here's how long every piece of the perfect #blog post should be:How Long Should Your Headlines Be? Your headline is one of the most important parts of your blog post. It’s what hooks your reader in, and if you don’t grab their attention from the get-go, you’ll lose them before you even begin. So where does the length of your headline come into play? Our research shows that headlines should be about 55 characters  to get the most click-throughs. To test your headlines before you publish your blog posts, use our  Headline Analyzer: How Long Should Your Sub-Headers Be? What’s a sub-header you ask? A sub-header is an H2 or H3 header tag in HTML that breaks up specific sections of each post. An H3 sub-head should be the sub-point of an H2 tag (and an H4 a sub-point of an H3, and so forth). Here at , we stick to H2 and H3-level headers. This helps maintain a clean and crisp appearance. So how long should a standard sub-header be? At we shoot for between eight to 10 words. It gives our readers enough to see there is a break in the section of the post without becoming overly wordy. Source: https://www.copyblogger.com/how-to-write-exquisite-subheads Keep sub-heads between 8 and 10 words long. Learn why (and get more content length tips) here:How Long Should Your Paragraphs Be? For web content writers a short paragraph is another crucial component of keeping your readers engaged with your content. So what’s the best length of a paragraph of text in your blog post? A maximum of three sentences. Why? Because it helps break down huge chunks of text which once again helps the reader consume your content and find the information they’re looking for. If you’re looking for some more insight into web content writing, read this post from Search Engine Journal. Recommended Reading: 40 Content Writing Tips to Make You a Better Marketer Now How Long Should Your Sentences Be? Just like short paragraphs, concise and readable sentences are important for your blog posts. Just like short paragraphs keep your blog post easy to read, so do short sentences. So how short should your sentences be? For easiest readability on the web, the answer is 25 words. Really long sentence have an overly abundant amount of words make it difficult to skim your content, and it becomes really easy to lose your train of thought. ^^See what I mean. Shorter sentences much easier to read. Keep sentences under 25 words for easy readability on the web.How Long Should Your Meta Descriptions Be? What is a meta description? A meta description is the short amount of text that appears below your web page in a Google search. It tells readers at a glance what kind of information is in your post. They look like this: Meta descriptions should be written around 156 characters or less. However, keep in mind that Google recently issued an update, and may display over 230 characters in some (but not all) cases. Summary: Here's How Long Everything in Your Blog Post Should Be We've thrown a lot of data and numbers at you. Feel like your head is spinning? Use this quick cheat sheet: Blog Post Content: 2,500 Words. Title Tag: 70 Characters. Meta Description: 156 Characters. Headlines: 55 Characters (or Six to Seven Words) Sub-Heads: Eight to 10 Words (Or Less) Sentences: 25 Words or Less. Paragraphs: Three Sentences or Less. There you have it! Now Go Write Great Blog Posts To sum up the studies from this post: Long form content is powerful if you want it to rank in Google. However, if your team is looking more for social shares or comments, shorter posts may be the better option for you. No matter what you decide, you need to look at your own data to see what your audience wants. Once you’re ready to start writing, create, edit and publish your blog posts with . Start a trial  or request a demo  today.

Parti5-comments2 Essay Example | Topics and Well Written Essays - 250 words

Parti5-comments2 - Essay Example A good cause and effect essay should move beyond establishing what the two parts are, and should also make an argument for why the cause and effect are directly related. The purpose of a cause and effect essay is to make an argument, not just to describe a process. The writer should prove the relationship of the cause and effect, and therefore have a thesis in the essay. The writer can’t just tell the reader the focus of the essay, because having a focus or purpose that is solely to explain a cause and effect will leave the reader disinterested. Overall, asking questions about the cause and effect process is a good way to develop the essay, but answering those questions persuasively is even more important. The reader dos not just want to know the reason why the effect happened is because of the cause, but also if there are alternative causes or alternative effects that may disprove the usual idea of any cause and effect. The essay should not just analyze or inform, but should convey an argument and prove to the reader that the cause and effect are

Data Analysis Skills for Economics and Accountancy Essay

Data Analysis Skills for Economics and Accountancy - Essay Example Similarly, a higher inflation can also signify that the economy is plunged into a debilitating recession, thus in both cases it is unclear to deduce which country has got a hand over the other. The above histogram describes the number of hours spent on average using a microcomputer in week by the users. Sample population comprises of 370 individuals which is enough to find the normality of the sample data. The histogram has a mean of 10.41 which implies that on average each user spends 10.41 hours in a week on the computer. The standard deviation of 8.993 which is very high and close to the mean value reveals that there are a lot of values which are deviating from the standard mean. These deviations are the outlier which can be confirmed by the maximum value (outlier) of 78. The data set has a range of 78 which implies that the difference between the maximum and minimum value of 78. The distribution of the above graph can not be certainly described as a normal one because it has too many outliers but it can be classified as skewed to left. After classifying the dataset into groups of 15 units we have divided the answers into categories ranging from very high to very low. Since the chi-square tests shows that the asymptotic significance value of 0.653 is greater than the significance level of 0.10 therefore we reach to a conclusion that gender does not affects the time spent by the

Aristotle Paper- Distinguishing the Definition Essay Example for Free

Aristotle Paper- Distinguishing the Definition Essay â€Å"A definition is an account, and every account has parts, and part of the account stands to part of the thing in just the same way that the whole account stands to the whole thing† (Aristotle 1034b20-22). This quote is how Aristotle defines a definition. So a definition is the statement of the essence of something. Defining something consists of starting with a genus and then breaking it down into species. A genus is a kind of a thing. A species is a more specific kind of something that is within a genus. Aristotle notices that something cannot be defined by its material components because each component can infinitely be broken down into more and more components(Aristotle 1035b9). Aristotle states the example- a circle can not be defined in terms of semicircles because semicircles would then have to be defined by quarter circles and so on, but a semicircle can be defined in terms of a circle because a circle is the simplest(Aristotle 1035b9). A genus is differentiated into species by dividing the genus into categories or certain types of the genus. Each of these categories or types within the genus are known as species. A species can also be a genus in that it too can be divided into specific categories or types. Those categories or types are also known as species, but can also be genus because they can further be divided into species. This pattern can go on infinitely. A ball is a substance that can be categorized into each setting of play that a ball is used on. So a ball can be categorized into something that is used to score on a soccer field, football field, baseball field, tennis court, basketball court, pool, and golf course. Each of these fields uses a specific type of ball. A golf course uses a golf ball. The definition of a golf ball is the ball that is used on a golf course. The essence of a golf ball is a ball. Work Cited Metaphysics. Trans. Hugh Tredennick. 2 vols. Loeb Classical Library 271, 287. Harvard U. -Press, 1933-35

Images, Imagery, Symbols, and Symbolism in Macbeth Essay -- Macbeth es

Imagery and Symbolism in Macbeth  Ã‚     Ã‚  Ã‚   With its eye-opening plot and interesting cast of characters, William Shakespeare’s play, Macbeth is one of the greatest works one could ever read. But, above all, the aspect of the play is most impressive and overwhelming with imagery and symbolism that Shakespeare so brilliantly uses. Throughout the play, the author depicts various types of imagery and symbolism instances that, eventually, lead to the downfall of the main character, Macbeth.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   Instances of imagery and symbolism are seen throughout the play. Imagery and symbolism are unavoidable features in William Shakespeare’s Macbeth. One of the most prominent symbolic factors in the play is the presence of blood. It has been noted that the presence of blood â€Å"increases the feelings or fear , horror , and pain† (Spurgeon , Pg. 20). From the appearance of the bloody sergeant in the second scene of the to the very last scene , there is a continued vision of blood all throughout the play. The imagery of blood seems to affect almost all the characters in the play. It affects Lady Macbeth in the scene in which she is found sleepwalking talking to herself after the murders of Duncan and Banquo : â€Å"Here’s the smell of the blood still. All the perfumes of Arabia will not sweeten this little hand.† Also , the blood imagery is present in the â€Å"weird sisters† , or witches. Most evidently , i t is present in act four, scene one, when Macbeth visits the witches to seek their insight and his fortune for the future. He is shown three apparitions , one of which is a bloody child that commands him to â€Å"Be bloody , bold and resolute : laugh to scorn†¦Ã¢â‚¬  Although blood imagery deals with almost all the characters of the play , no where i... ..., New York, Viking Publishing, 1993. Gove, Philip Babcock. Webster’s Third International Dictionary.   Springfield, Mass: G. & C. Merriam, 1967 Jorgensen, Paul A.   Our Naked Frailties.   Los Angeles: U of CA, 1971. Shakespeare, William. Macbeth. New York: Penguin Books, 1987. Shakespeare, William.   Tragedy of Macbeth . Ed. Barbara Mowat and Paul  Ã‚   Warstine. New York: Washington Press, 1992.  Ã‚  Ã‚   Staunten, Howard, The Complet Illustrated Shakespeare, New York, Park Lane Publishing, 1979. Steevens, George. Shakespeare, The Critical Heritage. Vol. 6. London:  Ã‚   Routledge & Kegan Paul, 1981.  Ã‚   Watson, Robert.   Thriftless Ambition, Foolish Wishes, and the Tragedy of Macbeth . Shakespeare and the Hazards of Ambition. Cambridge: Harvard UniversityPress, 1984.  Ã‚   Wills, Gary. Witches & Jesuits. Oxford: Oxford University Press, 1995.        

Archetypes in Beowulf Essay

Archetypes stir profound emotions in the reader because they awaken images stored in the collective unconscious. In Seamus Haney’s translation of Beowulf this is used in the form of character types. Beowulf is a hero and encounters many triumphs with different types of people on his journey in this epic poem. There are three archetypal characters in Beowulf that are particularly effective and intriguing. These are The Creature of Nightmare Grendel, The Mentor Hrothgar, and The Loyal Retainer Wiglaf. The characters are common experiences in the human psyche. The Creature of Nightmare is a monster from the deepest darkest part of the human psyche. Throughout Beowulf, Grendel’s actions and description accurately fits the archetype. The vivid language used in the poem illustrates Grendel as a monster. â€Å"The God-cursed brute was creating havoc:/greedy and grim he grabbed thirty men/from their resting places†¦/ (121-123)†. He’s also depicted as â€Å"a fiend out of hell/ (100)†. This passage acts as an illustration of the archetype in the poem because a demon that steals lives at a peaceful hour is what would happen in a nightmare. Grendel shares similarities with another dark creature from literature, Poseidon. He is known through Greek mythology as an almighty god but also is a monster. In the myth of Medusa, Poseidon took medusa’s virginity forcefully in the temple of Athena, had the blame put on her, and she was terribly punished. The acts he committed were iniquitous. What makes this archetype particularly effective and intriguing is the fact that these monsters are real; except they aren’t in the form of a nightmare or make believe gods. In addition to the Creature of Nightmare there is the Mentor. This individual acts as a teacher or consoler to the initiate. The fit for this archetype is Hrothgar. For the duration of the novel, he acts as role model to Beowulf consoling him to achieve greatness. Hrothgar depicts this archetype when he talks to Beowulf about life and how to be a good king. He says â€Å"†¦understand true values. / I who tell you have wintered into wisdom. / (1724-1725)† and â€Å"Do not give way to pride. / for a brief while your strength is in bloom but it fades quickly†¦Ã¢â‚¬ . Being a mentor requires experience so that wisdom can be bestowed onto another and that something is learned. He undoubtedly does this for Beowulf. Hrothgar can be compared to another character in literature. This character is Hagrid from  Harry Potter. It’s easy to overlook him but Hagrid turns out to be Harry’s rescuer and first real friend. He’s the first person to take Harry’s side in anything, and he is the first real impression that Harry gets of the wizard world, aside from the letters. Harry makes use of relying on friendship throughout all of the books thanks to Hagrid. These details make him an exemplary example of a mentor. The Mentor archetype is effective and intriguing because people still have mentors by their side in the form of a mother, father, friend, teacher, or other guiding them through life. Lastly, there is The Loyal Retainers. These people are very important as they are usually a sidekick who has a duty to protect and reflect the nobility of the hero. Beowulf’s sidekick is Wiglaf. He comes at the end of Beowulf and is the only warrior who stays and helps Beowulf defeat the dragon, â€Å"No help or backing was to be had then/ f rom his high-born comrades; that hand-picked troop/ †¦ ran for their lives†¦/but within one heart sorrow welled up/ (2597-2600)†. He also stays with Beowulf when his last breath is taken. Wiglaf gets this archetype because he protected the hero, Beowulf. He shares very similar qualities with Ponyboy from The Outsiders. He is the main character but when his friend, Johnny, kills someone Ponyboy runs away with him staying by Johnny’s side till he died even though he could have let Johnny get in trouble by himself. Ponyboy protected Johnny and stood by his side. The archetype, Loyal Retainer, is effective and intriguing because there’s always somebody that is there for another person in life to protecting them and be loyal. Archetypes are persuasive in the epic poem Beowulf. The Creature of Nightmare, The Mentor, and The Loyal Retainer are common experiences in the human psyche. These archetypes can be connected to other literature. They can also be connected to life. The ideas in this essay matters to the world because as according to Carl Jung people from all over the world respond to certain myths or stories in the same way not because everyone knows the same story but because lying deep in our collective unconsciousness are the racial memories of humanity’s past. These memorie s exist in the form of archetypes.

Regulatory Bodies

ROLE OF REGULATORY BODIES INTRODUCTION Health Professionals such as nurses doctors, Pharmacist and many others are regulated and licensed by regulatory bodies as required by provincial legislation. All nurses are required to be licensed to practice with their designated provincial nursing regulatory body. Legal responsibility in nursing practice is becoming of greater importance as each year passes. In order to provide safe and competent nursing care an understanding of legal boundaries is very essential. It is important to know the law in one state and the authorities enforcing these laws. VITAL ROLE OF REGULATORY BODIES * To ensure the public’s light to quality health care service. * To support and assist professional members. * Set and enforce standards of nursing practice. * Monitor and enforce standards for nursing education. * Monitor and enforce standards of nursing practice. * Set the requirements for registration of nursing professionals. Nursing regulatory bodies also known as colleges or associations, are responsible for the licensing of nurses with in their respective provinces territory. The Nursing Regulatory bodies receives their authority from legislation. MAJOR TYPES OF REGULATORY BODIES * The central government. * The state government * Institutional Rules * Trained Nurses Association of India * International council for Nurses * American Nurses Association * Canadian Nurses Association * National League for Nursing ROLE OF CENTRAL GOVERNMENT The central government is a source of regulatory body in three ways, through. 1) Government service conduct rules 2) The Indian Nursing council Act 3) The English law THE GOVERNMENT SERVICE CONDUCT RULES These are detailed rules of conduct for are government employees. Examples of these are the requirement to maintain absolute integrity, devotion to duty and high standards of moral behaviour. Only a few are applicable to the nursing practice, but all would be applicable to the practice of a nurse employed by the government. INDIAN NURSING COUNCIL ACT The Indian Nursing Council, which was authorised by the Indian Nursing Council Act of 1947, was established In 1949 for the purpose providing uniform standards in nursing education and reciprocity in nursing registration throughout the country. The only national legislation directly related to nursing practice, also provides a basis from which rules for nursing practice can be developed. Among other responsibilities, this Act gives authority to the Indian Nursing Council for prescribing curricula for nursing education and recognising qualifications of institutions with teaching programmes for nursing. This means that the INC has authority to control nursing education and what the nurse is prepared to do. It is important because legal responsibility does finally depend upon what you should be able to do and how you should do it as well as what you are not prepared to do. The INC uses this authority in nursing education but it delegates authority for control of nursing practice to the State Nurses’ Registration Councils. INDIAN NURSING COUNCIL The Indian Nursing Council was authorized by the Indian Nursing Act of 1947. It was established in 1949 to providing uniform standards in nursing education and reciprocity in nursing registration through out the country. Nurses registered in one stat were not necessarily recognized for registration in another state before this time. The Condition of mutual recognition by the state Nurses Registration Councils, which is called reciprocity, was possible only if uniform standards of nursing education were maintained. FUNCTIONS OF INC 1) It provide uniform standards of in nursing education and reciprocity in nursing registration. 2) It has authority to prescribe curriculum for nursing education in all states. 3) It has authority to recognize programme for nursing education or to refuse recognition of a programme if it did not meet the standards required by the council. ) To provide the Registration of foreign nurses and for the maintance of the Indian Nurses Register. 5) The INC authorizes State Nurses Registration Council and Examining Board to issue qualifying certificates. The INC has been given heavy responsibilities for nursing practice and nursing education but it has not been able to exert enough power to support high standards in nursing. ENGLISH LAW The law based upon the English Pattern is the third way in which the Central Government is a source of legal authority. These laws are very specific and make you â€Å"liable for negligence† or answerable to the laws for acts of carelessness. The laws summarised below are given for medical practitioners including nurses. 1) The right to refuse to the treat a patient expect in an emergency situation. 2) The right to sue for fees. (Applicable only to private duty nurse or private practitioners: other nurses are salaried. ) 3) The right to add a titile descriptions to one’s name. Any title, description, abbreviation or letter which implies nolding a degree, diploma, license or certificate showing particular qualifications may be added. (Improper use of these is often prohibited by State Nurses Registration Acts. The right to wear the Red Cross Emblem is given only to members of the Army medical service. 4) Unregistered practitioners are not allowed to hold positions or appointments in public and local hospitals 5) Fundamental duties. a) To exercise a reasonable degree of skill and knowledge in treating patients. The standard held is that exercised by other reputable members of the same profession in similar circumstan ces. b) Once a relationship to a patient has been established, there is an obligation to attend the patient as long as necessary unless the patient requests withdrawal or notice is given of intention to withdraw. ) A practitioner must give personal attention to his cases and answer calls with reasonable promptness. d) Children must be protected from harming themselves. e) Special precautions must be taken in the case of adults who are incapable of taking care of themselves. 6) The Indian Penal Code demands that poisonous drugs be kept in separate containers properly labeled and marked. Care must be taken not to mix with nonpoisonous drugs. 7) There is a duty of secrecy to the patients. Records must be treated as confidential unless the practitioner is called upon to give evidence in court. 8) Dangerous diseases must be reported. (Theses will vary in different parts of the country. ) 9) Nurses as considered solely responsible for their own professional acts irrespective of the employing authority. A fine is the usual penalty imposed for disobeying the law stated above, although imprisonment is also possible. The central responsibility consists mainly of Policy making, palnning, guiding, assisting evaluating and Co. ordinating the work.

24 Great Places to Buy Used Law Books Online

24 Great Places to Buy Used Law Books Online Law books are without a doubt one of the biggest expenses for students, and it doesnt help that textbook prices increased nearly threefold between 1986 and 2004 according to the  Government Accounting Office  (PDF). Sadly, selling them back for pennies is perhaps even more depressing than buying them in the first place. But gone are the days when students could only go to the school bookstore and perhaps one or two used bookstores off campus to collect all of their necessary supplies. The Internet has created a virtual playground for shoppers, and here are 28 places you can save money on law books and note that many buy back books as well (so perhaps you can get money back in the future!): AbeBooks.com: A subsidiary of Amazon.com with books discounted up to 90% off list price.AddALL: Popular textbook search and comparison engine. You can also try their ebook comparison engine at ebooks.adall.com.Alibris.com: Textbooks from 10,000 independent bookstores.Amazon.com: Surely you know of Amazons excellent book selection, but dont miss their Marketplace, which has some of the best prices anywhere on used books and ebooksBarnes Noble: Save up to 30% on new textbooks and 90% on used textbooks with free shipping on textbook orders of $25 or more.BnCTextbookRental.com: Shop by your course schedule and save 25% on used books through Barnes Noble College book rentals.Better World Books: Free shipping in the United States; sales help fund literacy projects in America and around the world.Biblio.com: Brings together over 5,500 independent booksellers for a selection of over 100 million books.BigWords.com: Probably the most popular textbook price comparison engine.Books-A-Million: Free shipping on orders over $25 and Millionaires Club for 10% discount on purchases. BookByte: Buy, sell, or rent law books and also get great deals on study guides and other materials.BookFBookFinder.com: 150 million books. 1 search engine.CampusBooks.com: Compare prices on law books and save up to 95% on textbooks and ebooks tooCampusBooks4Less.com: Search engine that price compares for you.CollegeBooksDirect.com: Promises same day shipping.CollegeSwapShop.com: Price comparison search engine.eBay.com: Lots of former students sell their law books directly on eBay and their companion site,  Half.com.eCampus.com: Award winning site where you can buy new and used books for up to 95% off with a bill me later option for no payments for 90 days.eTextShop.com: Buy and sell textbooks; guarantees the highest price when selling your books.MBS Direct: Partners with some schools to provide what it says is the largest inventory of new and used textbooks in America.Powells Books: Started on a derelict corner of Portland, Oregon in the early 1970s, Powells has withstood the test of time and now serves customers worldwide. Textbooks.com: Buy textbooks to 90% off with free shipping. Also, buys your textbooks for great prices.TextbookX.com: Nearly a million titles in stock and its own Facebook application to facilitate sales. Also, sells school supplies at low prices.ValoreBooks.com: Buy and sell used law books; offers great buy back prices. And here are two last bits of advice before you go online shopping for law books: Be sure you have the correct ISBN numbers for the editions of the books you need; and shop early for the best prices and books in the best condition. Happy shopping!

Profile of Pope Clement VI

Profile of Pope Clement VI Pope Clement VI is an important figure in Medieval History. Key Facts Pope Clement VI was also known as Pierre Roger (his birth  name). Accomplishments Sponsoring a naval crusading expedition, buying land for the papacy in Avignon, patronizing arts and learning,  and defending the Jews when pogroms flared up during the  Black Death. Occupation: Pope Place of Residence and Influence: France Important Dates: Born:  c.  1291Elected pope: May 7, 1342Consecrated: May 19, 1342Died:  1352 About Pope Clement VI Pierre Roger  was born in Corrà ¨ze, Aquitaine, France, and entered a monastery when he was still a child. He studied in Paris and became a professor there, where he was introduced to Pope John XXII. From then on his career took off; he was made  abbot of Benedictine monasteries at Fà ©camp and La Chaise-Dieu before he became archbishop of Sens and Rouen and then a cardinal. As Pope, Clement  was strongly pro-French. This would cause difficulties when attempting to broker peace between France and England, who were at that time engaged in the decades-long conflict that would come to be known as the Hundred Years War. Unsurprisingly, his efforts saw little success.   Clement was  the fourth pope to reside in Avignon, and the continued existence of the Avignon Papacy did nothing to lessen the problems that the papacy  had with Italy. Noble Italian families disputed the papacys claim to the territory, and Clement sent his nephew,  Astorge de Durfort, to settle matters in the Papal States. Though Astorge would not be successful, his use of German mercenaries to aid him would set a precedent in papal military matters that would last another hundred years. Meanwhile, the Avignon Papacy persisted. Not  only did Clement turn down an opportunity to return the papacy to Rome, but he also purchased Avignon from Joanna of Naples, whom he absolved of her husbands murder. Pope Clement  chose to stay in Avignon during the  Black Death and  survived the worst of the plague, though a third of his cardinals died. His survival may have been due, in large part, to his doctors advice to sit between two huge fires, even in the heat of summer. Though it wasnt the doctors intent,  the heat was so extreme that plague-bearing fleas couldnt get near him. He also offered  protection to the Jews when many were persecuted under suspicion of starting the pestilence. Clement  saw some success in crusading, sponsoring a naval expedition that took control of Smyrna, which was given to the Knights of St. John, and ended its pirate raids in the Mediterranean. Spurning the idea of clerical poverty, Clement  opposed extremist organizations like the Franciscan Spirituals, who advocated absolute rejection of all material comforts, and became  a patron of artists and scholars. To that end, he enlarged  the papal palace and  made it a sophisticated center of culture. Clement was a generous host and a magnanimous  sponsor, but his lavish spending would deplete the funds his predecessor, Benedict XII, had so carefully amassed, and he turned to taxation to rebuild the papacys treasury. This would sow the seeds of further discontent with the Avignon Papacy. Clement died in 1352 after a short illness. He was interred as per his wishes at the abbey at  La Chaise-Dieu, where 300 years later Huguenots would desecrate  his grave and burn his remains. More Pope Clement VI Resources Pope Clement VI in Print Clement VI: The Pontificate and Ideas of an Avignon Pope (Cambridge Studies in Medieval Life and Thought: Fourth Series) by Diana Wood Pope Clement VI on the Web Pope Clement VI, Substantial biography by N. A. Weber at the Catholic Encyclopedia.

Case study Essay Example | Topics and Well Written Essays - 250 words - 1

Case study - Essay Example 2) If I was the CEO at Coca-Cola, I would respond to concerns about the company by assigning a marketing team to help restore the image of Coca-Cola. This team would focus on presenting a positive side of Coca-Cola to the news media and also follow up any complaints immediately. The emphasis would be to create a friendly image to the public that represents Coca-Cola’s ethics and values. 3) I think that the social work that Coca-Cola is doing is great because many people do not even realize that it is going on. Maybe Coca-Cola needs to focus more on gaining recognition for its charitable works. Their heart is sincere so I don’t think that they are doing it to simply â€Å"look good.† Things like HIV/AIDS really make a connection with the average everyday person because these issues are the types of problems that people continually face. If Coca-Cola can maintain its environmentally-friendly image, then the company’s success should be in no